ObjectiveTo understand antimicrobial resistance and homology of carbapenemresistant Klebsiella pneumoniae (CRKP) from an intensive care unit (ICU).Methods11 CRKP isolates from patients and environment of an ICU in a hospital were performed antimicrobial susceptibility testing, the homology of CRKP was analyzed by randomly amplified polymorphic DNA (RAPD) method.ResultsOf 11 CRKP isolates, 8 were from patients, and 3 from environment, the main specimen was sputum(n=6, 54.55%). Antimicrobial susceptibility testing results revealed that 10 (90.91%) CRKP isolates were resistant to ciprofloxacin; 11 isolates were susceptible to compound sulfamethoxazole, intermediate to imipenem, and resistant to other antimicrobial agents(100%). All 11 CRKP isolates had 3 bands, and were divided into two types: (type Ⅰ, n=10; type Ⅱ, n=1).ConclusionAntimicrobial resistance of Klebsiella pneumoniae in ICU is serious, CRKP isolated from ICU patients and environment are of the same clone. Cleaning, disinfection, and monitoring of ICU environment should be strengthened, which is helpful for reducing, timely warning of multidrugresistant organisms, and reducing healthcareassociated infection.