ObjectiveTo investigate the detection of multidrugresistant organisms(MDROs) in a hospital, evaluate the efficacy of multidisciplinary team(MDT）on management of MDROs, and provide guidance for effective control on MDRO infection.MethodsFrom October 2013 to September 2014, compliance to comprehensive intervention measures in clinical departments in different stages as well as detection of MDROs from patients were compared respectively.ResultsCompliance to comprehensive intervention measures showed an overall upward trend from the fourth quarter of 2013 to the first, second, and third quarters of 2014, difference was statistically significant （all P＜0.001）.From the fourth quarter of 2013 to the third quarter of 2014, the percentage of the major MDRO strains in the same species of bacteria were: methicillinresistant Staphylococcus aureus(MRSA) 52.34%,45.45%, 48.95%, and 26.25% respectively；carbapenemresistant Acinetobacter baumannii （CRAB）64.42%,63.07%,59.87%,and 43.09% respectively；multidrugresistant Pseudomonas aeruginosa (MDRPA) 42.11%,41.82%,29.33%, and 17.52% respectively；the detection rate of MRSA,CRAB, and MDRPA showed an overall downward trend, difference among different stages were statistically significant （all P＜0.001）. Detection rates of carbapenemresistant Enterobacteriaceae (CRE) and vancomycinresistant Enterococcus (VRE) were both low (＜5%), difference among different stages were not statistically significant （all P＞0.05）.ConclusionMDT on management of MDROs is helpful for reducing the emergence and spread of MDROs.