ObjectiveTo detect bacterial content on surface of mobile phones of health care workers (HCWs) by adenosine triphosphate (ATP) bioluminescence assay.MethodsHCWs in departments of internal medicine, surgery, medical laboratory, and administration were randomly selected, 50 in each department, field detection on bacterial content on surface of mobile phones of HCWs was conducted, the relevant data were recorded.ResultsA total of 200 mobile phones were detected, 33 mobile phone surface were qualified, the qualified rate was 16.50%. Qualified rate of mobile phone surface of HCWs in different departments as well as mobile phone disinfected by different modes were different（χ2=13.46, 10.24, respectively，both P＜0.01）; difference in qualified rate of mobile phones of different types, different service life, and different protective case were all not significant （χ2=4.37,1.87, 0.25 respectively，all P＞0.05）.ConclusionThe qualified rate of bacterial content on surface of HCWs’ mobile phone is low, the awareness of hand hygiene of HCWs should be strengthened, regular cleaning and disinfection on the mobile phone can effectively reduce bacteria on the mobile phone surface.