ObjectiveTo evaluate the effect of comprehensive intervention measures on preventing and  controlling  surgical site infection (SSI) in patients undergoing total abdominal hysterectomy.MethodsPatients undergoing total abdominal hysterectomy in a hospital between January 2011 and December 2014 were investigated retrospectively, patients in 2011 were as control group, from January 2012, a prospective monitoring on SSI was initiated, comprehensive intervention measures were taken, patients between January 2012 and December 2014 were as intervention group, SSI before and after the intervention  was compared.ResultsA total of 1 052 patients undergoing total abdominal hysterectomy were investigated, 267 cases were monitored before intervention, 25 patients (9.36%) developed healthcareassociated infection (HAI), 12(4.49%) of whom were with SSI;  785 cases were monitored after intervention, 13(1.66%) of whom were with SSI, incidence of  SSI in  2012, 2013,and 2014  were  2.31%, 1.89%, and 0.77% respectively, there was a decreasing tendency（χ2=7.30, P＜0.01）.ConclusionProspective monitoring on SSI and comprehensive intervention can reduce the incidence of SSI in patients undergoing total abdominal hysterectomy.