ObjectiveTo understand the distribution of multidrugresistant organisms (MDROs) in a cancer hospital, and provide basis for the prevention and control of healthcareassociated infection (HAI).MethodsMonitored data of MDROs from clinical specimens in Guizhou Cancer Hospital in 2011-2013 were analyzed retrospectively.ResultsA total of 1 165 MDRO strains were isolated in 2011-2013, most of which were extendedspectrum betalactamaseproducing Escherichia coli (n=626,53.73%), followed by methicillinresistant coagulase negative staphylococcus (MRCNS, n= 209,17.94%); MDRO detection rate increased year by year. MDROs were mainly isolated from sputum/throat swabs（n=346,29.70%）, secretion（n=322,27.64%）, and urine（n=227,19.48%）; intensive care unit and department of urology were the major departments of emergence of MDROs, accounting for 18.03% (n=210) and 14.85% (n=173) respectively.ConclusionMDROs in this hospital are mainly isolated from respiratory tract, surgical wound, and urinary system, monitoring of highrisk departments and susceptible population should be strengthened, targeted prevention and control measures should be developed to curb the infection and spread of MDROs.