ObjectiveTo investigate the status of tuberculosis (TB) infection among health care workers (HCWs) in an infectious diseases hospital, and explore the risk and influencing factors of TB infection.MethodsAll HCWs in an infectious diseases hospital were surveyed through questionnaire and purified protein derivative (PPD) testing.ResultsIncidence of TB infection among all HCWs in this hospital was 48.18%. There was no significant difference in TB infection among HCWs in different departments（P＞0.05）. TB infection among HCWs of different working seniority, different ages, and different job titles were all significantly different （all P＜0.05）. Risk factors for TB infection were education level, job title, living condition, and working time in TB clinics or wards, OR (95%CI) were 1.70（1.03-2.80）, 1.95（1.10-3.45）,1.84（1.03-3.28）,and 2.38（1.40-4.04）respectively；personal protection was a protective factor for TB infection (OR,0.92 ［95% CI, 0.85- 0.99］）.ConclusionHCWs in infectious diseases hospital are at high risk of TB infection, they should improve their selfprotection consciousness, and take protective measures as early as possible.