ObjectiveTo study infection status of hepatitis C virus(HCV) in patients at a tertiary firstclass hospital in Hubei Province.MethodsDetection results of HCVIgG in hospitalized patients with nondigestive system diseases and patients in infectious diseases department between May 2012 and April 2014 were collected, HCV infection rates in patients of different genders and age groups were analyzed statistically.ResultsA total of 104 487 patients were conducted HCV detection, 1 619 (1.55%) were positive for HCV; HCV infection rates in male and female patients were 1.56% and 1.54% respectively, HCV infection rate was not significantly different between different genders, and different age groups（both P＞0.05）.ConclusionPositive rate of HCV in this study is lower than average national level, prevention and control of healthcareassociated infection caused by HCV should be intensified.