ObjectiveTo evaluate the effect of comprehensive intervention measures on preventing and controlling the infection/colonization of multidrugresistant organism(MDRO) in respiratory tract of intensive care unit(ICU) patients.MethodsThroat swabs and sputum specimens from artificial airway suction were taken for bacterial culture and antimicrobial susceptibility testing at the first, fourth, eighth day, and then every seven day of patients’ admission. JuneDecember 2013 was as preintervention group, from January 2014, comprehensive intervention measures began to be implemented, JuneDecember 2014 was as postintervention group, detection of MDROs were analyzed statistically.ResultsA total of 253 isolates of MDROs were detected, before and after intervention were 164 and 89 isolates respectively, 191 isolates（75.49%） were carbapenemresistant Acinetobacter baumannii strains, before and after intervention were 119 and 72 isolates respectively. Incidence of MDRO respiratory tract infection before and after intervention were 52.94% and 30.43% respectively, difference was significant(χ2=5.02，P=0.03）. The average hospitalization time of patients in pre and postintervention groups were (8.07±2.52) days and (6.89±1.71） days respectively, difference was significant （t=5.40，P＜0.01）.ConclusionMDROs are major pathogens causing healthcareassociated infection (HAI) in ICU patients, comprehensive intervention measures can reduce the occurrence of HAI.