ObjectiveTo investigate the status and change trend of healthcareassociated infection (HAI) in a tumor hospital, so as to provide reference for formulating scientific control measures of HAI and strengthening management of HAI.MethodsAccording to the requirement of the National HAI Surveillance Network, on September 19, 2012, August 20, 2013 and August 13,2014, survey on HAI prevalence rates among all hospitalized patients in this hospital were carried out, data were analyzed statistically.ResultsThe prevalence rates of HAI from 2012 to 2014 were 6.93%,8.82%, and 8.10% respectively；lower respiratory tract was the main infection site in three years, accounting for 30.00%, 53.85%, and 47.44% respectively；followed by upper respiratory tract, abdominal (pelvic) tissue, superficial incision, and urinary tract. Gramnegative bacilli were the main pathogens causing HAI, accounting for 71.43%, 66.67%,and 72.97% respectively, the detection rate of Escherichia coli was the highest. Antimicrobial usage rates in 2012-2014 were 18.23%,13.64%, and 17.70% respectively；pathogenic detection rates in patients receiving therapeutic antimicrobial use were 74.51%,57.14%,and 55.68% respectively（χ2=15.122，P=0.001）. Antimicrobial usage rates in type Ⅰ incisional operation were 0.78%, 6.25%, and 8.33% respectively.ConclusionSurvey of HAI prevalence rate is helpful for realizing the current situation of HAI and deficiency of prevention and control of HAI, taking targeted measures, and reducing incidence of HAI.