ObjectiveTo investigate the effect of implementation of hand hygiene(HH) activities on HH compliance among health care workers(HCWs).MethodsIn MayDecember 2013, a series of HH intervention measures in a hospital were implemented, compliance to HH among all clinical and laboratory HCWs in AprilDecember 2013 (before and after intervention) were surveyed and compared.ResultsAfter implementing HH activities, HH compliance rate of HCWs increased from 39.82% before intervention to 80.77% after intervention, HH compliance rates of doctors, nurses and cleaning staff increased from 41.09%,41.30% and 34.33% before intervention to 83.87%,91.89%, and 59.54% after intervention respectively， the differences between before and after intervention were all statistically significant (all P<0.001). Except intensive care unit, HH compliance rates of HCWs in departments of surgery, internal medicine, and radiotherapy were all higher than preintervention(all P<0.001). Compliance to five indications for HH among HCWs after intervention were all higher than preintervention(all P<0.001), HH  compliance rate before touching a patient increased from 23.88% to 73.37%，before clean/aseptic procedure increased from 58.65% to 94.23%.ConclusionThrough the implementation and supervision of HH activities, both the awareness and compliance of HH among HCWs have been significantly improved, good HH habit is gradually developed.