ObjectiveTo evaluate the effect of medical hygiene wipes on disinfecting object surface and compliance to use in an intensive care unit (ICU). Methods30 beds in the ICU at a hospital were randomly selected and divided into three groups: A, B and C, each group had 10 beds. Group A were only provided hygiene wipes, group B used towel soaked in chlorinecontaining disinfectant (disinfected towel), group C were  provided both medical hygiene wipes and disinfected towel for selected use. Compliance to different disinfection methods, and disinfectant efficacy on object surface were compared.ResultsThe rate of compliance to disinfection in group A was higher than group B ( 78.78% vs 42.12%, χ2=68.56，P＜0.05）；except medical disposal trolley and ventilator,  compliance to disinfection of the other object surface in group A were all higher than group B ( all P＜0.05）.Except ventilator, adenosis triphosphate (ATP) values before and after disinfection of object surface in group A and B  were both significantly different (both P＜0.05）, disinfectant efficacy of group A was superior to group B.  Group C used medical hygiene wipes more than disinfected towel（［836  times，91.57%］ vs ［77 times，8.43%］）.Nurses spent (20.15+ 3.00) minutes on preparing disinfectant every day, and （15.02±2.00）minutes  on washing  used disinfected towel, while  hygiene wipes could be used immediately, discarded after use, and was timesaving. ConclusionUse of medical hygiene wipes is convenient, rapid,and without irritating odor,  it also improves disinfection effect on object surface and ICU health care workers’  compliance to disinfection