ObjectiveTo investigate nursing interns’ knowledge and attitude towards needle stick injury before clinical practice. MethodsIn 2023 June,2015, nursing interns who were about to start clinical practice in a hospital were investigated, nursing interns’ knowledge and attitude towards needle stick injury were surveyed through questionnaire. ResultsA total of 350 questionnaires were distributed, 324 (92.57%) responded questionnaires were available. 40（12.35%）questionnaires were responded by male interns，and 284（87.65%）were by female interns；34（10.49%）interns had bachelor degree；the mean age of interns were (20.83 + 1.24) years old. The correct answer rates about questions related to injury occurring during needle recapping and wearing gloves were low (about 60%). About 70% of the interns gave the correct answers to questions about hepatitis B infection due to needle stick injury following hepatitis B vaccination, as well as medication after injury. Score for individual question about attitude towards needle stick injury was ≤3,  the major related problems were susceptibility of  blood borne diseases and recapping needles.ConclusionNeedle stick injuryrelated knowledge and attitude among nursing interns is inadequate, including recapping needles, timely report, susceptible to infectious diseases, and so on. It is necessary for schools and teaching hospitals to strengthen the education about occupational protection among nursing interns, so as to improve the attitude and ability of professional protection.