ObjectiveTo evaluate the basic situation of healthcareassociated infection（HAI）in hospitals in different regions of Guizhou Province, so as to provide basis for making HAI prevention and control measures.MethodsFrom January 1 to September 30, 2014, 118 secondary or above level medical institutions in 9 regions of Guizhou Province were investigated through the use of questionnaires, data about prevalence rate of HAI, antimicrobial use, surgical site infection(SSI), and deviceassociated infection were summarized and analyzed.ResultsA total of 52 531 patients were surveyed, 1 175 cases of HAI occurred, prevalence rate of HAI was 2.24%, antimicrobial usage rate in 51 735 patients was 39.02%, microbiology specimen submission rate in patients receiving therapeutic antimicrobial agents was 38.81%. Postoperative pulmonary infection and SSI rates among 8 119 patients were 0.71% and 2.50%  respectively. Targeted monitoring data showed the rates of catheterassociated urinary tract infection, ventilatorassociated pneumonia, and central venous catheterrelated bloodstream infection were 2.56 ‰, 7.62 ‰,and 1.12 ‰ respectively. Differences in specimen submission rate in patients receiving therapeutic antimicrobial agents, postoperative pulmonary infection rate, and SSI rate among different regions were statistically significant (χ2 =1 128.459, 16.086, 31.360，respectively, all P<0.05).ConclusionThere remain some differences in postoperative pulmonary infection, SSI, deviceassociated infection, and specimen submission rates among different regions. HAI monitoring, prevention, and control measures should be developed for all levels of medical institutions.