ObjectiveTo understand the cleaning status of hospital environment, and evaluate the effect of fluorescence labeling method plus feedback and training on hospital environmental cleaning effectiveness.MethodsA total of 27 departments in a hospital were investigated, 1 cleaning staff and 2 inpatients were selected from each department, cleaning staff’s knowledge about cleaning and disinfection of environmental object surfaces, as well as cleaning status of inpatients’ wards were surveyed, cleaning efficacy of hospital environmental object surfaces were detected with fluorescence labeling method, the surveyed results were performed timely feedback to clinical departments, training on cleaning and disinfection knowledge was conducted, the effective cleaning rate of environmental object surface before and after the training was compared.ResultsA total of 27 cleaning staff were surveyed, the correct response rate for cleaning frequency was 96.30%，awareness rate for section concept was 96.30%，accuracy rate of cleaning order was 92.59%，accuracy rate of postcleaning immersion time of sanitary wares in disinfectant was 85.19%，accuracy rates of replacing, drying, and repeated immersing wiping cloths were 81.48%，48.15%，and 25.93% respectively， rates of correct disinfectant formulating method and mop drying time were both 0. Among 54 investigated patients, bed units and ground of wards of 28 patients were cleaned both 1-2 times /day; bed units of 8 patients had never been wiped, 18 patients in 9 departments cannot be conducted statistics due to completely inconsistent responses with the other patients of the same departments. The effective cleaning rates of environmental object surfaces before and after the training were 34.62% and 64.96% respectively，difference was significant（χ2=21.81，P＜0.01）.ConclusionFluorescence labeling method plus feedback and training can improve cleaning efficacy of hospital environmental object surfaces.