ObjectiveTo explore the distribution and antimicrobial resistance of pathogens causing healthcareassociated infection(HAI) in a hospital, and provide theoretical basis for rational use of antimicrobial agents and control of HAI.MethodsHAI occurred in patients in this hospital between 2011 and 2015 were chosen, distribution of major pathogens causing HAI and antimicrobial resistance were analyzed.ResultsA total of 852 pathogenic strains were isolated, including 458 (53.75%) strains of gramnegative bacteria, 259 (30.40%) fungi, and 135(15.85%) grampositive bacteria, the top 4 pathogens were Klebsiella pneumoniae(n=159，18.66%), Candida albicans (n=119，13.97%), Acinetobacter baumannii(n=81，9.51%), and Escherichia coli(n=74，8.69%). Isolation rate of gramnegative bacteria increased from 47.41% in 2012 to 61.57% in 2015, fungi increased from 24.78% in 2011 to 34.45% in 2014 (2015 was 27.95%), grampositive bacteria decreased from 26.55% in 2011 to 10.48% in 2015. Resistance rates of Escherichia coli and Klebsiella pneumoniae to imipenem and meropenem were 8.11%-12.58%；Acinetobacter baumannii had the lowest resistance rate to cefoperazone/sulbactam(23.46%)，resistance rate of Candida albicans to fluconazole was up to 59.66%，vancomycinresistant grampositive bacteria was not found.ConclusionThe major pathogens causing HAI are opportunistic pathogens, antimicrobial resistance is serious, the species and distribution varies with different years, it is necessary to enhance specimen detection rate, strengthen the monitoring on pathogen resistance, and use antimicrobial agents rationally.