ObjectiveTo investigate the distribution of pathogens and positive alarming time of blood culture, and provide basis for laboratory diagnosis and clinical treatment. MethodsBlood specimens from clinical departments in a hospital in MayNovember 2015 were collected, positive alarming time of blood culture was recorded, species of pathogens were identified. ResultsA total of 157 pathogenic strains were isolated from blood culture specimens, grampositive cocci, gramnegative bacilli, and fungi accounted for 31.85%, 57.32%, and 10.83% respectively. The median positive alarming time were as follows: Enterobacteriaceae 0.50 day, nonfermenting bacteria 0.63 day, Enterococcus spp. 0.60 day, Streptococcus spp. 0.80 day, Staphylococcus spp. 1.01 days, and fungi 1.44 days, respectively. ConclusionPositive alarming time of blood culture specimens from early to late are as follows: Enterobacteriaceae, Enterococcus, nonfermentative bacteria, Streptococcus spp., Staphylococcus spp., and fungus. Positive alarming time of pathogens causing bloodstream infection are all within 4 days, and most of them are within 1 day.