ObjectiveTo observe air disinfection efficacy of ultraviolet ray, ozone disinfector, and circulating air disinfector in outpatient blood collection room.MethodsAir in outpatient blood collection room was disinfected by ultraviolet ray, ozone disinfector, and circulating air disinfector, air specimen was taken before and after air disinfection, as well as after 1, 2, 3, and 4 hour working condition of staff, efficacy of 3 disinfection methods were compared.ResultsUnder static condition, there was no significant difference in average colony forming unit(CFU)  among 3 disinfection methods（P=0.317），the average CFU in air which disinfected by ultraviolet ray and ozone disinfector increased rapidly after the entry of personnel, the number of air colony did not meet the requirements of category Ⅲ environmental sanitation standard after staff worked for 2 hours; circulating air disinfector could continuously disinfect after the entry of personnel, the average CFU met the requirement of category Ⅲ environmental sanitation standard after staff worked for 1, 2, 3, and 4 hours; there were significant difference in the CFU among different disinfection methods（F=211.00，P＜0.001），there were significant difference in CFU at different sampling time（F=272.95，P＜0.001）.ConclusionCirculating air disinfector can meet the requirements of category Ⅲ environmental sanitation standard during the working condition of staff, and is suitable for air disinfection in outpatient blood collection room.