ObjectiveTo evaluate the cleanliness of frequently touched object surfaces in a hospital and efficacy of intervention measures.MethodsCompliance to cleaning of frequently touched object surfaces before and after intervention was surveyed by fluorescence labeling method, SPSS 17.0 statistical software was used to analyze data.ResultsBefore and after intervention, 6 800 items in 400 wards were investigated, compliance rates to cleaning of hospital object surfaces before and after intervention were 14.71% and 54.76% respectively（P＜0.001）; differences in compliance rates to cleaning of object surfaces in common wards and special wards before and after intervention were both statistically significant（both P＜0.001）；after intervention, compliance rates to cleaning of object surfaces in wards and toilets increased significantly compared with before intervention, which increased by 41.57% and 33.00% respectively，differences were statistically significant (both P＜0.001）; after intervention, compliance rates to cleaning of different object surfaces increased by 21.50%-52.00% (all P＜0.001）.ConclusionScientific and effective intervention measures can improve the cleaning effectiveness of frequently touched object surfaces, which can improve the environmental quality of hospital.