ObjectiveTo study the homology characteristics of clinicaly isolated and colonized linezolid（LZD）resistant Enterococcus faecalis (E. faecalis ) strains from a patient.MethodsTen E. faecalis  strains (2 were isolated from urine specimens and 8 were from stool specimens) isolated from a patient with pulmonary infection were performed antimicrobial susceptibility testing, homology of E. faecalis  was determined by pulsedfield gel electrophoresis (PFGE).ResultsBefore and after patients received LZD therapy, 2 E. faecalis  strains isolated form urine specimens were both resistant to LZD (MICs: 8 mg/mL, 16 mg/mL, respectively),  among 8 strains from stool specimens (6 were isolated before therapy, and 2 were isolated after therapy), LZD susceptible, intermediate, and resistant strains were 4, 2, and 2 respectively（MICs: 0.25-12 mg/mL）. 10 strains of E. faecalis  were homologous by PFGE typing. ConclusionIn this case, the detection of E. faecalis  from urinary tract and intestinal tract is homologous, which suggested that LZDresistant Enterococcus may be colonized in vivo for a long time, and may be shift to cause bacterial infection.