ObjectiveTo investigate the distribution and antimicrobial resistance of bacteria isolates from intensive care units(ICUs) in Shandong Province．MethodsAntimicrobial susceptibility data about clinical strains isolated from ICUs of 101 hospitals and general wards(nonICUs) of 106 hospitals in Shandong Province in 2014 were analyzed statistically．ResultsA total of 24 458 strains of bacteria were isolated, of which gramnegative and grampositive bacteria accounted for 80.37% and 19.63% respectively．The top five pathogens in ICUs were Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus. Resistance rates of Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, and Serratia marcescens obtained from ICUs to carbapenems, piperacillin/tazobactam, cefoperazone/sulbactam, and amikacin were all higher than those from nonICUs（all P＜0.01）. Resistance rates of Acinetobacter baumannii, Pseudomonas aeruginosa, and Staphylococcus spp. from ICUs were all higher than those from nonICUs（P＜0.05）. Resistance rates of Acinetobacter baumannii isolated from ICUs to imipenem and meropenem were 78.0% and 79.0% respectively；while Pseudomonas aeruginosa were 25.5% and 21.6% respectively, resistance rates to other antimicrobial agents were all <30.0%. Isolation rate of methicillinresistant Staphylococcus aureus from ICUs was higher than that from nonICUs（39.7% vs 26.0%, P＜0.05）.ConclusionAntimicrobial resistance of bacteria isolated from ICUs is serious, rational use of antimicrobial agents should be guided according to the results of antimicrobial susceptibility testing result, so as to prevent the spread of drugresistant bacteria.