ObjectiveTo investigate the prevalence rates of healthcareassociated infection(HAI) and communityassociated infection(CAI) in hospitalized patients in Wuhan City, and provide a scientific basis for the prevention and control of HAI. MethodsConvenience sampling method was used to select 33 hospitals in Wuhan City, all hospitalized patients were surveyed by bedside investigation and medical record reviewing, SPSS 16.0 software was used for data analysis. ResultsA total of 36 222 hospitalized patients were investigated, of whom 1 116 (3.08%) had HAI, 6 968 (19.24%) had CAI. The prevalence rate of HAI was highest in hospitals with ≥900 beds（3.40%）, and the prevalence rate of CAI was highest in hospitals with＜300 beds （43.70%）. Of departments, general intensive care unit had the highest prevalence rate of HAI(32.88%), department of respiratory diseases had the highest prevalence rate of CAI (78.34%). A total of 699 pathogens were isolated from patients with HAI, the top three were Pseudomonas aeruginosa (18.03%), Acinetobacter baumannii (16.31%) and Staphylococcus aureus (12.88%), a total of 1 149 pathogens were isolated from patients with CAI, the top three were Escherichia coli (14.45%), Pseudomonas aeruginosa (11.23%), and Mycoplasma (10.01%). The main infected sites of both HAI and CAI were the lower respiratory tract, accounting for 48.24% and 45.15% respectively. ConclusionHAI and CAI have different characteristics, it is necessary to take targeted measures according to key departments and key sites, so as to reduce the occurrence of HAI effectively.