ObjectiveTo understand the direct economic burden of healthcareassociated infection(HAI) due to multidrugresistant organisms(MDROs). MethodsComputer retrieval of CNKI, Wanfang, VIP, PubMed, ScienceDirect, and Cochrane databases on literatures about economic burden of MDRO HAI at home and abroad were performed, the retrieval time was from database establishment to December 2015, systematic evaluation of the literatures was obtained. ResultsAccording to the inclusion and exclusion criteria, as well as through NewcastleOttawa Scale (NOS) for evaluating the literatures, 19 literatures were included. In 12 studies about methicillinresistant Staphylococcus aureus infection, the direct economic cost varied from $916.61 to $62 908.00; in 4 studies about MDRO Acinetobacter baumannii infection, the direct economic cost varied from $4 644.00 to $98 575.00. Direct economic cost due to extendedspectrum βlactamasesproducing Enterobacteriaceae was $2 824.14-$30 093.00. ConclusionMDRO HAI will increase economic cost of both hospitals and patients, prevention and control measures should be taken .