ObjectiveTo investigate the current status of cleaning and disinfection of digestive endoscopes in the endoscope centers (rooms) of secondary and above medical institutions in Suzhou City, find the existing problems and put forward suggestion for improvement. MethodsOnthespot survey on current status of cleaning and disinfection management for digestive endoscopes in 28 medical institutions(18 tertiary medical institutions, 10 secondary medical institutions) was performed, endoscopes were selected and detected with ATP biofluorescence assay after cleaning and disinfection. ResultsAll 28 surveyed medical institutions had separate endoscope disinfection rooms, 89.29% of which had integrated endoscopic cleaning station, 17.86% had automatic endoscope washer/disinfector; 100% used multienzymatic detergent, chose the right disinfectant, monitored disinfectant concentration every day, and implemented standard disinfection time. But only 39.29% changed multienzymatic detergent for each endoscope, cleaning and disinfection personnel in 78.57% of medical institutions wore personal protective equipment correctly. 77 digestive endoscopes were detected, the qualified rate was 88.31%. ConclusionCleaning and disinfection management of digestive endoscope in secondary and above medical institutions in Suzhou City is generally standardized, there are still some problems in the manipulation procedures, relevant national regulations should be strictly complied with, efficacy of cleaning and disinfection of digestive endoscope should be further improved.