ObjectiveTo understand the current situation and characteristics of occupational exposure(OE) among health care workers(HCWs), and provide basis for making preventive measures. MethodsSelfdesigned questionnaire about OE was adopted, OE among all HCWs in a hospital in JanuaryDecember 2015 were surveyed and statistically analyzed. ResultsA total of 1 888 HCWs were surveyed, there were 304 cases of OE, the mean OE rate in the whole hospital was 16.10%; OE occurred 370 times(19.60%). Doctors, trainees，and HCWs who worked for≤1 year had relatively higher OE rates, which were 23.18%(102/440), 17.88%(49/274), and 18.34%(95/518) respectively. Sharp injury is the main OE mode, accounting for 83.24%（n=308）, sharp injuries mainly occurred before disposing the used devices and during the process of use, accounting for 37.99%(n=117) and 36.69%(n=113) respectively. Among 370 times of OE, 315 could be traced to the sources of exposure. Among the known exposure sources, OE of bloodborne pathogens accounted for 24.13% (n=76). 59 of 370 times of OE implemented complete local treatment and reporting procedures, reporting rate of OE was 15.95%. ConclusionHospital should take comprehensive measures to promote occupational safety precaution, and reduce the incidence of OE.