ObjectiveTo investigate the prevalence of healthcareassociated infection(HAI) in a tumor hospital, and provide evidence for prevention and control of HAI. MethodsAccording to requirement of cross sectional survey of nationwide HAI monitoring network, prevalence rates of HAI in hospitalized patients at a tumor hospital in 20132015 were surveyed, surveyed results were statistically analyzed. ResultsA total of 3 515 hospitalized patients were investigated from 2013 to 2015, 24 (0.68％) had HAI. The prevalence rates of HAI from 2013 to 2015 were 0.79％, 0.54％, and 0.76％ respectively, difference was not statistically significant(χ2＝0.65, P＞0.05). The main infection site was lower respiratory tract, accounting for 45.83%. The main pathogens causing HAI were gramnegative bacilli, accounting for 47.37%, followed by fungi(26.32%). ConclusionThe prevalence rate of HAI in tumor patients is low, targeted monitoring should be carried out according to the current situation, the prevention and control of lower respiratory tract infection should be focused on, fungal infection should be paid attention.