ObjectiveTo understand the basic situation of healthcareassociated infection(HAI) management and prevention and control of multidrugresistant organism(MDRO) infection in medical institutions in Hubei Province. MethodsQuestionnaires were used to investigate the basic situation of HAI management and MDRO infection in 47 tertiary and secondary hospitals in Hubei Province.Results47 hospital were enrolled in this study, HAI management fulltime staff was allocated with a median of 0.90/250 beds; in 2015, the isolation rates of methicillinresistant Staphylococcus aureus, carbapenemresistant Enterobacteriaceae, carbapenemresistant Pseudomonas aeruginosa, carbapenemresistant Acinetobacter baumannii, and vancomycinresistant Enterococcus were 39.13%, 4.13%, 19.44%, 63.60%, and 2.77% respectively. 47 hospitals all carried out surveillance on MDROs, 28（59.57%） hospitals were installed HAI monitoring system software, could directly obtain data through collecting with the laboratory system. The diagnosis of MDRO infection was mainly based on the combined diagnosis by HAI fulltime personnel and clinicians(26 hospitals, 55.32%）. 44 (93.62%) hospitals regularly convened coordination meeting on prevention and control of MDROs, in 2015, hand hygiene compliance rate were 10.0%-89.2% by selfinspection; 42 (89.36%) hospitals routinely prescribed isolation orders for patients with MDRO infection, 33 (70.21%) hospitals conducted environmental clean and disinfection twice a day, 24 (51.06%) hospitals performed personal protection by using complete set of protective equipment. ConclusionThis investigation is helpful for understanding the general situation of HAI management in medical institutions, as well as the detection and control of MDROs in this area, and make continual improvement on the problems and weakness found in the investigated project, so as to promote the development of HAI management in this area.