ObjectiveTo understand the distribution and antimicrobial resistance of pathogens isolated from patients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD) in Ordos area, so as to provide guidance for rational antimicrobial use.MethodsSputum culture and antimicrobial susceptibility testing results of 372 patients with AECOPD in Ordos area in 20132015 were analyzed, and the quality of life before acute exacerbation was assessed.ResultsA total of 296 strains of pathogens were isolated from 372 patients, 252(85.14%) of which were gramnegative bacteria, the major were Pseudomonas aeruginosa(n=58, 19.59%), Klebsiella pneumoniae(n=51, 17.23 %), Acinetobacter baumannii(n=50, 16.89%), Stenotrophomonas maltophilia(n=22, 7.43%), Escherichia coli(n=19, 6.42%), and Enterobacter cloacae(n=16, 5.41%); 27(9.12%) were fungi, the major was Candida albicans(n=19, 6.42%); 17（5.74%） were grampositive bacteria, the predominant species was Staphylococcus aureus. Patients with CAT (COPD assement test) score≥10 had higher proportion of isolating Pseudomonas aeruginosa and Acinetobacter baumannii than those with CAT score<10. ConclusionThe main pathogens from patients with AECOPD are gramnegative strains, CAT score prior to exacerbation may be related to the emergence of pathogens at AECOPD.