ObjectiveTo investigate the effectiveness of training at morning shift meeting in improving healthcareassociated infection(HAI) prevention and control among health care workers(HCWs) in newly established hospital departments. MethodsOn July 11-22, 2016, HAI management fulltime personnel performed training for HCWs about HAI prevention and control at morning shift meeting of 8 newly established clinical departments and 1 laboratory, the questionnaire survey was conducted before, immediately after, and 3 months after training, training effectiveness was evaluated. ResultsA total of 239 HCWs were required to receive training, including 85 doctors (35.6%), 150 nurses (62.8%), and 4 technicians (1.7%). The knowledge of HAI prevention and control that most HCWs wanted to know was occupational exposure and occupational precaution（85.6%）；most HCWs wanted to obtain knowledge of HAI through training in their respective department by HAI management fulltime personnel（87.1%）. Total awareness rates of HAI knowledge before, immediately after, and 3 months after training were 45.1%, 96.7%, and 83.9% respectively, difference was significant(P<0.001); comparison among groups showed that HCWs’ awareness in influencing factors of HAI, hand hygiene opportunity, isolation measures, and medical waste classification 3 months after training was significantly lower than immediately after training（all P<0.001）；the average scores of HAI prevention and control knowledge among HCWs immediately after and 3 months after training were both higher than before training(P<0.001); average score of HAI prevention and control knowledge among HCWs 3 months after training was lower than that of immediately after training（P<0.001）. ConclusionThe training at morning shift meeting of clinical departments can improve the awareness of HAI prevention and control knowledge among HCWs.