ObjectiveTo study the changing trend of prevalence rates of healthcareassociated infection (HAI) and antimicrobial use in hospitalized patients in medical institutions of Hubei Province, and provide a scientific basis for improving HAI management.MethodsThe crosssectional survey results of HAI in Hubei Province in 2010, 2012, and 2014 were analyzed. ResultsThe prevalence rates of HAI in 2010, 2012, and 2014 were 3.48%（1 526/43 909）, 3.03%（1 919/63 320）, and 2.86%（2 174/76 145）respectively, which showed a downward trend, difference was statistically significant（χ2=36.44，P<0.01）. Antimicrobial usage rate decreased from 54.29% (23 838/43 909) in 2010 to 41.02% (31 238/76 145) in 2014, difference was statistically significant(χ2=2 194.09, P<0.01). Among patients receiving therapeutic use of antimicrobial agents, the specimen detection rate increased from 30.49% (4 297/14 091) in 2010 to 52.13% (10 556/20 248) in 2014 (χ2=1 593.98, P<0.01). ConclusionThe prevalence rate of HAI showed a downward trend in Hubei Province, crosssectional survey on antimicrobial use showed a gradual decrease.