ObjectiveTo investigate the distribution characteristics of methicillinresistant Staphylococcus aureus (MRSA) in a children’s hospital, and provide basis for the prevention and control of MRSA infection in children. MethodsChildren who admitted to a children’s hospital from 2011 to 2015 were analyzed retrospectively, clinical data of children, isolation of pathogens, types of specimens, and healthcareassociated infection(HAI) status were analyzed. ResultsFrom 2011 to 2015, a total of 911 children isolated Staphylococcus aureus (SA, 1 108 positive specimens), 494 of whom isolated MRSA (599 positive specimens), 54.23% of children isolated MRSA（isolation rate of specimens was 54.06%）；there was no significant difference in the isolation rate of MRSA between children of different genders（P＞0.05）；isolation rate of MRSA in different age groups was statistically significant（P＜0.05）. Isolation rates of MRSA from blood, puncture fluid, secretion, and pus were 68.97%, 66.00%, 55.81%, and 54.47% respectively. Isolation rate of SA and MRSA increased from 0.61% and 21.74% in 2011 to 1.40% and 75.59% in 2015 respectively, difference were both significant(both P<0.05). Incidence of SA and MRSA increased from 0.198% in 2011 to 2.697% and 2.119% in 2015 respectively, both showed an upward trend year by year(both P<0.05). ConclusionIsolation rate of MRSA and incidence of HAI in this children’s hospital increased year by year, it is necessary to intensify management, use antimicrobial agents scientifically and rationally, timely perform disinfection and isolation, so as to curb the emergence and spread of MRSA in hospital settings.