ObjectiveTo evaluate the effect of three methods on detecting cleaning efficacy of suction luminal devices in pediatric department, and find out a proper detection method for such devices. MethodsRandom number table method was adopted to take samples of suction lumen devices after cleaned in central sterile supply department in JanuaryApril 2016, visual observation with the aid of magnifying glass, jieli strip detection method, and ATP bioluminescence assay were adopted to detect cleaning efficacy of outer surface, screwed connection, and inner wall of lumen devices, the qualified rates of cleaning detected by three methods were compared.  ResultsThe qualified rates of suction lumen devices after cleaned detected by visual observation with the aid of magnifying glass, jieli strip detection method, and ATP bioluminescence assay were 99.00%, 91.00%, and 71.50% respectively，qualified rates of cleaning detected by three methods were statistically significant（χ2 =71.545，P＜0.001）, pairwise comparison showed a significant difference (both P＜0.0167）； qualified rates of cleaning of the outer surface of lumen detected with three methods were all 100.00%； qualified rates of cleaning of screwed connection were 100.00%, 98.00%, and 97.00% respectively（χ2=5.695，P＞0.05）；qualified rates of cleaning of inner wall were 99.00%, 92.00%, and 72.00% respectively（χ2=72.634，P＜0.01）, pairwise comparison were all significantly different（all P＜0.01）. ConclusionThe cleaning quality of the outer surface and screwed connection of pediatric suction lumen devices can be assessed by visual observation with the aid of magnifying glass, cleaning quality of the inner wall of lumen can be detected by ATP bioluminescence method, when condition is limited，Jieli strip method can be used for routine detection, supplemented by ATP bioluminescence detection on a regular basis.