ObjectiveTo explore the role of infection control nurses in healthcareassociated infection(HAI) management, and provide basis for HAI management. MethodsThrough setting up infection control nurses in clinical departments of the whole hospital, clarifying their responsibilities and duties, training, and supervising them, the effect of infection control nurses on HAI management was observed. ResultsA total of 67 infection control nurses were set up in the clinical departments of the whole hospital, HAI management knowledge among health care workers (HCWs) in 26 departments improved significantly, scores of HAI management knowledge among HCWs in April and December was compared, difference was statistically significant (Z=-2.193，unilateral P=0.014）. Hand hygiene compliance rate of HCWs improved from 83.35%（1 817/2 180）in April to 89.53% (2 002/2 236) in December, difference was statistically significant（χ2=36.13，P＜0.01）. A total of 56 670 hospitalized patients were monitored from April to December 2015, the total length of hospital stay was 411 164 days, utilization rate of three catheters was 27.18%, three catheterrelated infection rate was 0.74‰. The median scores of supervision on HAI management in clinical departments improved from 95.30 in May to 97.70 in September（P＜0.05）. ConclusionSetting up infection control nurses is of great significance to strengthen the HAI management organization and promote the quality of HAI management.