ObjectiveTo compare bacterial blocking rate of different medical packaging material, as well as weight loss rate, tear strength, and abrasion resistance of cotton cloth with different times of washing. MethodsAccording to test methods for sterile medical device packaging, bacterial blocking rates of cotton cloth, nonwoven fabric, and disposable filter paper were tested. Weight loss test, tensile test, and abrasion resistance test of cotton cloth were performed to test weight loss rate, tear strength, and abrasion resistance of cotton cloth with different times of washing. ResultsIn test for microbial barrier ranking of porous package material, the average bacterial blocking rates of doublelayer new cotton cloth, doublelayer old cotton cloth, disposable filter paper, and nonwoven fabric were 62.15%, 31.39%, 82.04%, and 89.71% respectively; after cotton cloth was washed 30 times, the weight loss rate decreased by 1.22%, tear strength decreased by 6.70%, fabricrubbing test revealed there was no pilling. ConclusionBacterial blocking effect ranked as follows: nonwoven fabric, disposable filter paper, doublelayer cotton cloth. After 30 times of washing, the weight loss rate and tear strength decreased, and bacterial blocking effect was worse than that of new cotton cloth.