ObjectiveTo evaluate the effect of personnel activities and air purifiers on airborne microorganisms and particulate matter in bronchoscopy room.MethodsAccording to whether there was personal activity and air purifier in the bronchoscopy room, the experiment was divided into four groups：dynamic nonpurification group, dynamic purification group, static nonpurification group, and static purification group, indoor air samples were collected and analyzed at five different time points (0, 0.5, 1, 2, 4 h), microorganisms in the air were collected by planktonic method, then cultured and counted, concentration of particulate matter was determined by DT9881M laser dust particle counter, variance analysis of factorial design was used for statistical analysis. ResultsColony count/concentration of airborne bacteria, fungi, total microorganisms (bacteria+fungi), PM2.5, and PM2.5-10.0 in dynamic nonpurification group were (113.53±7.78) CFU/m3，(89.67±7.17) CFU/m3，(203.20±10.92) CFU/m3，(86 557.20±4 158.29) counts/m3，and (659.69±38.91) counts/m3 respectively, in static nonpurification group were (84.33±3.65) CFU/m3, (65.00±2.65)CFU/m3, (149.33±4.98) CFU/m3, (45 812.64± 1 279.61) counts/m3, and (189.15±4.64) counts/m3 respectively, in dynamic purification group were (84.80±8.08) CFU/m3，(90.40±5.50) CFU/m3，(175.20±9.22) CFU/m3，(49 336.38±2 039.16) counts/m3, and (218.36±7.02) counts/m3 respectively，in static purification group were (67.80±5.63) CFU/m3, (38.27±3.70) CFU/m3, (106.07±6.76) CFU/m3, (29 772.53±2 212.93) counts/m3, and (124.80±7.16) counts/m3 respectively. Colony count/concentration of airborne bacteria, total microorganisms, PM2.5, and PM2.5-10.0 in dynamic group were all higher than those in static group, nonpurification group were higher than purification group(both P<0.05)，colony count of fungi in dynamic nonpurification group was higher than static nonpurification group, in static purification group was lower than static nonpurification group(both P<0.05)，there was no significant difference between dynamic purification group and dynamic nonpurification group （P=0.936）.ConclusionPersonal activities can increase colony count/concentration of microorganisms and particulate matter in bronchoscopy room, air purifier can reduce the bacteria, total microbial count, and particulate matter in the air of bronchoscopy room.