ObjectiveTo study the bactericidal efficacy of hospital commonly used disinfectants such as ethanol, 3" chlorine tablets, iodophor, glutaraldehyde, and avagard instant hand antiseptic on Staphylococcus aureus(S. aureus) from different sources of hospital, and provide scientific basis for effective control of healthcareassociated infection(HAI).MethodsA total of 48 strains of S. aureus from inpatients, hands of health care workers, and environment surface in the First Affiliated Hospital of Nanchang University were collected. Disinfectant was directly contacted with bacteria, in vitro killing efficacy of disinfectant on S. aureus from different sources at different diluted concentrations, and different contact time were studied.ResultsThe killing rate of 5g/L iodophor, 20g/L glutaraldehyde, and avagard instant hand antiseptic(0.5% chlorhexidine+70% ethanol) to S. aureus with a 5minute contact time was 100%; killing rates of 70% ethanol and 1g/L 3" chlorine tablets to S. aureus with a 5minute contact time were 96.5%-99.8%；but highly diluted iodophor, glutaraldehyde, and avagard instant hand antiseptic still could not completely kill S. aureus even the contact time was extended.ConclusionThe routine use of disinfectants in the hospital can meet the clinical bactericidal efficacy, it is necessary to monitor concentration routinely, avoid decreasing sterilization ability.