ObjectiveTo investigate the distribution and drug susceptibility of vaginal pathogens in pregnant women during perinatal period.MethodsVaginal discharge specimens of 10 800 women (5 400 were pregnant women during perinatal period, 5 400 were nonpregnant women) were performed bacterial culture and drug susceptibility testing. ResultsThe isolation rate of pathogens from 5 400 perinatal pregnant women was 26.00% (n=1 404), including 759 strains of fungi, 611 strains of grampositive cocci, 32 strains of gramnegative bacilli, 2 strains of Neisseria gonorrhoeae; among 5 400 nonpregnant women, the isolation rate of pathogens was 7.87% (n=425), including 232 strains of fungi, 182 strains of grampositive cocci, 5 strains of gramnegative bacilli, and 6 strains of Neisseria gonorrhoeae. Resistance rates of Streptococcus agalactiae isolated from perinatal pregnant women to erythromycin and clindamycin were 84.85% and 80.81% respectively，resistance rate of Staphylococcus aureus to erythromycin was 40.91%, resistance rates of Escherichia coli to tetracycline and sulfamethoxazole/trimethoprim were 69.23% and 53.85% respectively, resistance rate of Neisseria gonorrhoeae to penicillin and sulfamethoxazole/trimethoprim was 100.00%. ConclusionVaginal infection rate in perinatal pregnant women is higher than nonpregnant women, screening of vaginal pathogens in perinatal pregnant women should be strengthened, so as to ensure the safety of mothers and infants.