ObjectiveTo explore the effect of SixSigma management on prevention and control of catheterrelated bloodstream infection(CRBSI) in intensive care unit(ICU). MethodsPatients with indwelling central venous catheter in the ICU of a hospital in MarchDecember 2015 were selected, five phases of SixSigma(define, measure, analyze, improve, and control) were used to find out the key factors that affected CRBSI, targeted improvement measures were taken, incidence of CRBSI before and after adopting SixSigma was compared. ResultsAfter SixSigma management was adopted, incidence of CRBSI declined from 15.95‰（13/815） to 6.19‰(4/646), difference was significant(P＜0.05). ConclusionSixSigma management can reveal the defect in workflow, guide researchers to propose corresponding measures, and effectively reduce the incidence of CRBSI in ICU.