ObjectiveTo understand infection status, clinical and epidemiological features of respiratory tract infection(RTI) with human Bocavirus（HBoV） in children in Wuhan area. MethodsA total of 968 hospitalized children with acute RTI between January and December 2016 were recruited in the study. Sputum specimens were collected by sterile negative pressure suction method. HBoV, respiratory syncytial virus, adenovirus, A/Btype influenza virus, and parainfluenza virus I/II/III were detected, the prevalence and clinical features of HBoV infection were analyzed. ResultsAmong 968 cases, 75(7.75%) were positive for HBoV. The detection rates of HBoV in boys and girls were 5.68% and 2.07% respectively, there was no significant difference in the detection rate of HBoV between different genders（χ2=2.083，P=0.140）；53 (70.67%) HBoVpositive children were<1 year old, 63（84.00%） were<3 years old, there was significant difference in the detection rate among all age groups(χ2=4.60，P=0.043）. The detection rates in spring, summer, autumn, and winter were 9.78%, 5.29%, 2.86%, and 5.20% respectively； except September and October, HBoV was found positive in the other months, detection rate was highest in March (33.33%）. 17 cases were simple HBoV infection and 58 were mixed infection, the main clinical diagnosis of HBoV was bronchopneumonia（n=26, 34.67%）. The main clinical features of HBoV cases were fever（81.33%）, cough（77.33%）, and wheezing (77.33%). ConclusionHBoV is one of the important pathogens causing RTI in hospitalized children in Wuhan area, HBoV infection can occur throughout the year , the peak season are spring and summer, there is no difference between different genders. The most common clinical diagnosis of HBoV in hospitalized children is bronchopneumonia, the main manifestations are fever, coughing, and wheezing.