ObjectiveTo understand the occurrence of healthcareassociated infection(HAI), distribution of pathogens, and drug resistance in a general hospital in 2014-2016, provide basis for prevention and control of HAI. MethodsClinical data of hospitalized patients from January 2014 to December 2016 were collected by prospective and retrospective investigation, distribution and drug resistance of pathogens causing HAI were statistically analyzed. ResultsFrom 2014 to 2016, 4 750 patients had 5 352 cases of HAI, incidence and case incidence of HAI were 2.19% and 2.46% respectively. Incidences of HAI in three years were 2.47%, 2.07%, and 2.05% respectively, showing a decreased tendency, difference was statistically significant (χ2=36.217，P＜0.01). Incidences of HAI were high in intensive care unit, department of neurosurgery, as well as department of burn and plastic surgery, the common HAI sites were respiratory tract, urinary tract, and surgical sites. The main pathogens causing HAI were gramnegative bacteria (76.10%). Resistance rates of Escherichia coli to cephalosporins and fluoroquinolones were relatively higher (>60%); resistance rates of Klebsiella pneumoniae to carbapenems were relatively higher; resistance rates of Pseudomonas aeruginosa to carbapenems showed a increased tendency year by year （χ2=15.175，P=0.001）；antimicrobial resistance rates of Acinetobacter baumannii were all>50%. Methicillinresistant Staphylococcus aureus(SA) accounted for about 60% of SA, methicillinresistant coagulase negative Staphylococcus(CNS) accounted for more than 80% of CNS, vancomycin and linezolidresistant Staphylococcus spp. were not found. ConclusionThe common pathogens causing HAI in this hospital are higher. Scientific monitoring on HAI and regular analysis of clinical data are of great significance for guiding rational use of antimicrobial agents, controlling multidrugresistant organisms, and reducing the occurrence of HAI.