ObjectiveTo evaluate the effect of plandocheckact (PDCA) cycle management method on improving multidrugresistant organisms (MDROs) prevention and control measures. MethodsPDCA cycle management method was applied to improve the management system and management process of a hospital, prevention and control efficacy of MDROs in clinic departments between JulyDecember 2014 (before PDCA implementation) and JanuaryJune 2015 (after PDCA implementation) was compared. ResultsAfter half a year implementation of PDCA cycle management, the total implementation rate of MDROs prevention and control measures increased from 57.14% (360/630) to 79.69% (510/640), difference was statistically significant(P<0.05); implementation rate of recording by doctors during the disease course , recording by nurses during the nursing process, registration and reporting of MDROs, as well as posting isolation signs were all improved, difference was statistically significant (all P< 0.05). The total isolation rate of MDROs declined from 30.65%（347/1 132）before PDCA implementation to 22.61%（236/1 044） after PDCA implementation, difference was statistically significant（χ2=10.393， P＜0.05). ConclusionApplication of PDCA cycle can improve the prevention and control measures of MDROs.