ObjectiveTo understand the drug resistance of Mycobacterium tuberculosis(MTB) and susceptibility of multidrugresistant MTB (MDRMTB) to linezolid in Hebei Province, so as to guiding clinical treatment of MDR tuberculosis.MethodsThe isolated strains and clinical information of patients with tuberculosis in 6 hospitals of 5 cities in Hebei Province between January and December 2016 were collected, susceptibility of MTB to antituberculous drugs isoniazid (INH), rifampicin（RFP）, streptomycin (SM), ethambutol（EMB）, ofloxacin（OFX）, and kanamycin（KM）were detected, 100 strains of MDRMTB were selected by stratified random sampling method, susceptibility to linezolid was detected.ResultsDrug resistance rate and MDR rate of the initially treated cases were 26.6%（200/753）and 13.5%（102/753） respectively, drug resistance rate and MDR rate of the retreatment cases were 59.7%（132/221）and 53.4（118/221）respectively, drug resistance rate and MDR rate of the retreatment cases were both statistically higher than initially treated cases（χ2=83.7，P＜0.01; χ2=93.5，P＜0.01）. Resistance rates of MTB to firstline antituberculous drugs INH, RFP, SM, and EMB were 25.8%, 23.7%, 16.7%, and 7.1% respectively, to secondline antituberculous drugs OFX and KM were 4.7%（37/782）and 4.0%（31/782） respectively；susceptibility of MDRMTB to linezolid was 80.8% (59/73).ConclusionDrug resistance rate and MDR rate of the retreated tuberculosis patients are higher than initially treated patients, linezolid has good in vitro antimicrobial activity against MDRMTB.