ObjectiveTo investigate the effect of business process reengineering(BPR) on improving multisectors’ participation in management of multidrugresistant organism(MDRO) infection, and provide methodological guidance for hospital multisectors’ collaborative management. MethodsRelated data about management and disposal of 672 cases of MDRO infection occurred from July 2015 to June 2017 were selected, 370 patients before BPR (from July 2015 to June 2016) were as control group, 302 patients after BPR (from July 2016 to June 2017) were as a trial group, BPR was used to improve the process of detection, report, cooperation, and disposal of MDROs in hospital, various quality evaluation indexes of healthcareassociated infection before and after BPR were compared. ResultsAfter the BPR was implemented, time of MDRO information transmitted from laboratory to clinical departments shortened from （240±30）minutes to （8±2）minutes; incidence of MDRO HAI decreased from 2.39‰ to 1.56‰ , isolation rate of MDROs decreased from 13.42% to 11.09%，differences were all significant(all P<0.05). Compliance rates and awareness rates of various MDRO prevention and control measures increased from 58.11%-71.89% to 84.11%-92.05%, usage rate of antimicrobial agents decreased from 53.18% to 48.45%, defined daily doses (DDDs) of antimicrobial use density decreased from 44.76 to 38.26, specimen submission rate before antimicrobial use increased from 46.68% to 53.62%. ConclusionBPR can enhance the cooperation between different departments, give full play to the complementary advantages of interdisciplinary, and improve the efficiency of HAI management.