ObjectiveTo evaluate disinfection efficacy of slightly acidic hypochlorous water (SAHW) on object surfaces of hemodialysis room. Methods30 dialysis units in the hemodialysis room were divided into two groups(trichloroisocyanuric acid group and SAHW group) by random number table method, 15 dialysis units in each group. Trichloroisocyanuric acid group disinfected object surface of each hemodialysis unit with trichloroisocyanuric acid containing 500 mg/L available chlorine, and SAHW group disinfected with SAHW. The colony counts on object surface after 4 hour disinfection were detected, the qualified status and killing of multidrugresistant organisms(MDROs) between two disinfection methods were compared. ResultsAfter bedrails, screens of dialysis machine, and knobs of dialysis machine were disinfected by trichloroisocyanuric acid containing 500 mg/L available chlorine and SAHW respectively, the qualified rates of trichloroisocyanuric acid group were 90.00%,80.00%, and 90.00% respectively, SAHW group were 100.00%, 96.67%, and 100.00% respectively, difference was not significant between two disinfection methods(both P>0.05). SAHW disinfection testing showed that the killing rates of SAHW to four MDROs were both 100%. ConclusionDisinfection efficacy of SAHW is the same as that of trichloroisocyanuric acid containing 500 mg/L available chlorine, and has high killing effect on the common MDROs in the hemodialysis room.