ObjectiveTo understand the clone correlation of carbapenemresistant Acinetobacter baumannii (CRAB) in the environment of intensive care units(ICUs) in Guangzhou City, identify genotypes, and provide basis for prevention and control of healthcareassociated infection(HAI).Methods39 strains of CRAB isolated from environment of ICUs in 7 hospitals in Guangzhou City were collected, susceptibility to 10 kinds of antimicrobial agents was detected by KirbyBauer method, OXA gene of strains was detected by polymerase chain reaction (PCR), clone polymorphism analysis was performed with pulsedfield gel electrophoresis (PFGE) and multilocus sequence typing (MLST).ResultsAmong 39 strains of CRAB, resistance rate to levofloxacin was the lowest(56.4%), resistance rates to other 9 antimicrobial agents were all>90%. PCR results showed that 39 strains(100%) of CRAB all carried OXA51 gene, 37(94.9%) carried OXA23 gene, but OXA24 and OXA58 genes were not found. PFGE showed that 38 CRAB strains were divided into 5 clones, group A was the main epidemic clone, MLST analysis showed that the main clone of CRAB was ST195.ConclusionTransmission of CRAB clone carrying OXA23 gene exists in the ICU environment of Guangzhou City, cleaning and disinfection of ICU environment should be intensified, so as to reduce HAI caused by CRAB.