ObjectiveTo improve the cleanliness of object surface frequently touched by doctors and nurses through collaboration of multidisciplinary team(MDT）. MethodsIn the fourth quarter of 2015, a baseline survey was performed by healthcareassociated infection(HAI) management professionals in a hospital (baseline survey stage), in the first quarter of 2016, HAI management office combined with medical affairs office and nursing department to carry out collaboration of MDT intervention (intervention stage). The second, third, and fourth quarters of 2016 were evaluation stages for efficacy of implementation of intervention measures, cleanliness of frequently touched object surface at different stages was compared.ResultsIn the baseline survey, the qualified rates of fluorescent labeling in doctor group and nurse group were 44.25% and 61.61% respectively, after MDT joint intervention, the qualified rates of fluorescence labeling in doctor group and nurse group increased to 89.83% and 87.50% respectively, difference was statistically significant(both P<0.05). The incidence of HAI in baseline survey stage was 1.22%, at stage three of postintervention, incidence of HAI was 1.02%, difference was statistically significant (P<0.05). ConclusionMDT can effectively integrate hospital management resources, make medical affairs office and nursing department give full play to their respective function, and effectively enhance the cleanliness of frequently touched object surface during medical care.