ObjectiveTo observe the efficacy of a medical disinfectant ultrasonic coupling agent on the killing of five clinically isolated multidrugresistant organisms(MDROs).MethodsFrom March 2016 to May 2017, a disinfection ultrasonic coupling agent containing active ingredient, including triclosan and propylene glycol, was used to conduct carrier quantitative germicidal test on five clinically isolated MDROs, the killing efficacy to MDROs was observed.ResultsAfter 1.5, 3.0, and 4.5 minute disinfection time，the killing logarithms values of disinfection ultrasonic coupling agent to five MDROs (multidrugresistant Acinetobacter baumannii ［MDRAB］, methicillinresistant Staphylococcus aureus ［MRSA］, multidrugresistant Pseudomonas aeruginosa ［MDRPA］, carbapenemresistant Klebsiella pneumoniae ［CRKP］,  and extendedspectrum βlactamase Escherichia coli ［ESBLsEC］) were all＞3.0.ConclusionMedical disinfection ultrasonic coupling agent can effectively kill five common MDROs, and can take the place of disinfectant during ultrasonic examination.