ObjectiveTo evaluate the effectiveness of intervention measures for disinfection of lead clothes in orthopedic surgery. MethodsCleaning and disinfection efficacy, as well as application of lead clothes in operating room in an orthopedic hospital before and after the implementation of intervention measures were investigated and compared. Results488, 499, and 487 pieces of lead clothes were investigated before, three months after, and six months after intervention, application of lead clothes were 1 840, 1 901, and 1 801 times respectively. Before and three months after intervention, 12.55% and 0.26% of lead clothes were found visible blood stains respectively, difference was statistically significant(χ2=238.99，P=0.00）；six months after the intervention, 0.11% of lead clothes were found to be stained with blood, there was no significant difference compared with three months after the intervention（χ2=1.13，P=0.29）. Before and three months after intervention, the qualified rates of bacterial colony on lead clothes were 72.34% and 89.16% respectively, difference was statistically significant（χ2=171.24，P=0.00）；six months after intervention，qualified rate of bacterial colony on lead clothes was 90.62%, difference was not statistically significant compared with three months after the intervention （χ2=2.14，P=0.14）. Before and three months after intervention, rate of repeated use of lead clothes were 19.29% and 9.84% respectively, difference was statistically significant（χ2=70.08，P=0.00）；after six month intervention, rate of repeated use of lead clothes was 6.22%, which was significantly different from that after three month intervention（χ2=16.31，P=0.00）. ConclusionIntervention measures for lead clothes disinfection in department of orthopedic surgery can effectively improve the cleanliness of lead clothes, and the continuous effect of intervention measures is better.