ObjectiveTo explore infection characteristics and antimicrobial resistance of Burkholderia cepacia(BC) from two hospitals in Hainan Province, and compare their resistance to BC. Methods59 infected hospitalized patients who isolated BC in two hospitals between January 2012 and December 2016 were analyzed retrospectively, basic characteristics of infection, antimicrobial susceptibility results and difference in resistance of strains were analyzed. ResultsThe detection rates of BC from sputum specimen (55.94%) and intensive care unit (18.65%) were the highest. In both hospitals, most patients with BC infection were with underlying cardiovascular diseases, which were 11 cases and 13 cases respectively; BC from one hospital had higher susceptibility rates to ceftazidime, levofloxacin, and compound sulfamethoxazole, all were＞90%, while BC from the other hospital had resistance rate of>50% except for levofloxacin (resistance rate 34.62%, intermediate resistance rate 15.38%), difference in susceptibility rates of BC to ceftazidime, levofloxacin, and compound sulfamethoxazole were all statistically significant between two hospitals(all P<0.05). ConclusionMost patients with BC infection are accompanied by underlying diseases; antimicrobial resistance of BC in different hospitals is different.