ObjectiveTo investigate bacterial contamination of dressing change trolley before and after dressing change. MethodsOnsite survey method was adopted, a dressing change trolley in the plastic and burn ward in a hospital was taken specimens from March 23, 2016 to June 26, 2016 and from August 8, 2017 to September 13, 2017, respectively. The handle, the drawer handle and the top layer of dressing change trolley were taken specimens before and after dressing change trolley was used, as well as when it was at a resting state for 3 hours after disinfection. Specimens were performed bacterial culture, number of bacterial colonies per unit area was calculated, difference in the number of colonies at different specimen taken opportunities was compared. ResultsA total of 303 specimens were collected, including 90 specimens before dressing change, 123 specimens after dressing change, and 90 specimens at a resting state for 3 hours after disinfection. There was a significant difference in the number of bacterial colonies on the top layer of dressing change trolley at different specimen taken opportunities (P=0.003)；group comparison showed that the number of bacterial colonies on the top layer increased after dressing change than before dressing change and at a resting state for 3 hours after disinfection (both P<0.05). There was no significant difference in the number of bacterial colonies on the handle and drawer handle of dressing change trolley at different dressing change opportunities (all P>0.05). There was no significant difference in the number of bacterial colonies among different parts of dressing change trolley at the same specimen taken opportunities (all P>0.05). The qualified rates of three parts of dressing change trolley were all 100.00%, qualified detection rates of handle, drawer handle, and top layer of dressing change trolley were 100.00%, 97.56%, and 97.56% respectively，qualified detection rates of three parts at a resting state for 3 hours after disinfection were all 100.00%. ConclusionSurface disinfection can meet the hygienic standard for disinfection for class II surface health standards in hospitals in China, it is basically safe to use the dressing change trolley within 3 hours after disinfection.