ObjectiveTo analyze the relationship between multilocus sequence typing(MLST) and sources of specimens as well as antimicrobial susceptibility of Candida albicans(C. albicans), and explore the relationship among strains and their molecular epidemiological characteristics. MethodsC. albicans isolated from neonatal department in a hospital from April 2015 to May 2015 were collected, in vitro susceptibility testing was carried out by a kit, strains were typed by MLST, homology and molecular epidemiological characteristics of strains were analyzed. ResultsA total 15 C. albicans strains were isolated from 4 children and 1 health care worker, these strains were divided into 3 sequence types (ST) through MLST, 13 of which were ST 1997, and the other 2 were ST 1359 and ST 1933 respectively. Phylogenetic analysis of strains was carried out by eBURST software. ST 1997, ST 1359, and ST 1933 were in Group 13， Group 1，and Group 20 respectively, C. albicans isolated from peripheral blood and peripheral venous catheter of the same patient belonged to the same clone. ConclusionThere is an outbreak of C. albicans bloodstream infection in the neonatal department, corresponding preventive and control measures should be formulated to strengthen the management of healthcare associated infection in neonatal department.